Fabrication of protein chip by magnetic force.
J Nanosci Nanotechnol
; 9(12): 7104-7, 2009 Dec.
Article
em En
| MEDLINE
| ID: mdl-19908737
This research describes a new immobilizing method of many kinds of biomaterials (enzyme, antibody, and DNA) on a transducer array using magnetic force interaction as the short-range force. The method composes two immobilizing steps. In the first step, same biomaterials are immobilized on metal particles. In the second step, the particles are arranged by the fluidic self-assembly method at random on an array. An array immobilized many kinds of the particles become multichannel biosensor. The biosensor can apply to DNA chip, protein chip, multienzyme electrode, and so on. The metal particles and the array were fabricated by micromachining manufacture. The metal particles were multilayer structure (gold, titanium, and nickel). In the array case, sidewalls of patterning nickel dots on an array were covered by thick negative photoresist (SU-8), and the array was magnetized. The array and the particles were mixed in buffer solution, and were arranged by magnetic force interaction. A quarter of total nickel dots were covered by the particles. The binding direction of the particles was controllable, and condition of particles was almost with gold surface on top. The immobilization of the biomaterials to metal particles was able to materialize it by using 3-CPD. This confirmed an activity by the luminol radiation.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas
/
Nanotecnologia
/
Análise Serial de Proteínas
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article