[Production and characteristics of monoclonal antibodies to the diphtheria toxin].
Bioorg Khim
; 35(5): 618-28, 2009.
Article
em Ru
| MEDLINE
| ID: mdl-19915639
ABSTRACT
Monoclonal antibodies to the diphtheria toxin were produced without cross reactivity with the thermolabile toxin (LT) from Escherichia coli; ricin; choleraic toxin; the SeA, SeB, SeE, SeI, and SeG toxins of staphylococcus; the lethal factor of the anthrax toxin; and the protective antigen of the anthrax toxin. A pair of antibodies for the quantitative determination of the diphtheria toxin in the sandwich variation of enzyme-linked immunosorbent assay (ELISA) was chosen. The determination limit of the toxin was 0.7 ng/ml in plate and 1.6 ng/ml in microchip ELISA. The presence of a secretion from the nasopharynx lavage did not decrease the sensitivity of the toxin determination by sandwich ELISA. The immunization of mice with the diphtheria toxin and with a conjugate of the diphtheria toxin with polystyrene microspheres demonstrated that the conjugate immunization resulted in the formation of hybridoma clones which produced antibodies only to the epitopes of the A fragment of the diphtheria toxin. The immunization with the native toxin caused the production of hybridoma clones which predominantly produced antibodies to the epitopes of the B fragment.
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Base de dados:
MEDLINE
Assunto principal:
Toxina Diftérica
/
Anticorpos Monoclonais
/
Especificidade de Anticorpos
Limite:
Animals
Idioma:
Ru
Ano de publicação:
2009
Tipo de documento:
Article