Mutasynthesis of lincomycin derivatives with activity against drug-resistant staphylococci.
Antimicrob Agents Chemother
; 54(2): 927-30, 2010 Feb.
Article
em En
| MEDLINE
| ID: mdl-19917754
ABSTRACT
The lincomycin biosynthetic gene lmbX was deleted in Streptomyces lincolnensis ATCC 25466, and deletion of this gene led to abolition of lincomycin production. The results of complementation experiments proved the blockage in the biosynthesis of lincomycin precursor 4-propyl-L-proline. Feeding this mutant strain with precursor derivatives resulted in production of 4'-butyl-4'-depropyllincomycin and 4'-pentyl-4'-depropyllincomycin in high titers and without lincomycin contamination. Moreover, 4'-pentyl-4'-depropyllincomycin was found to be more active than lincomycin against clinical Staphylococcus isolates with genes determining low-level lincosamide resistance.
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Base de dados:
MEDLINE
Assunto principal:
Staphylococcus
/
Streptomyces
/
Lincomicina
/
Antibacterianos
Limite:
Humans
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article