Mechanism of intestinal secretion. Effect of serotonin on rabbit ileal crypt and villus cells.
J Clin Invest
; 87(2): 743-6, 1991 Feb.
Article
em En
| MEDLINE
| ID: mdl-1991855
ABSTRACT
To determine the mechanism of action of an intestinal secretagogue, serotonin, we have isolated crypt and villus cells and demonstrated NaH and ClHCO3 exchange activity using the intracellular pH-sensitive fluorescent dye, 2,7-bis (carboxy-ethyl)-5,6-carboxy-fluorescein. Serotonin alkalinized both crypt and villus cells. Alkalinization in villus cells was HCO3 dependent and Na independent. In contrast, alkalinization in crypt cells was HCO3 independent and Na dependent. In villus cells, recovery from an alkaline load induced by Cl removal, 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid or propionate pulse, known to occur via the ClHCO3 exchange, is inhibited by serotonin. In contrast, in crypt cells, recovery from an acid load induced by Na removal, amiloride and NH4Cl pulse, known to occur via NaH exchange, is stimulated by serotonin. These data suggest that serotonin is inhibiting ClHCO3 exchange in villus cells and stimulating NaH exchange in crypt cells. These effects of serotonin would be expected to inhibit coupled Na and Cl absorption by villus cells and stimulate HCO3 secretion by crypt cells in the intact ileum.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Serotonina
/
Íleo
/
Microvilosidades
Limite:
Animals
Idioma:
En
Ano de publicação:
1991
Tipo de documento:
Article