GMab-1, a high-affinity anti-3'-isoLM1/3',6'-isoLD1 IgG monoclonal antibody, raised in lacto-series ganglioside-defective knockout mice.
Biochem Biophys Res Commun
; 391(1): 750-5, 2010 Jan 01.
Article
em En
| MEDLINE
| ID: mdl-19944071
ABSTRACT
The lacto-series gangliosides 3'-isoLM1 and 3',6'-isoLD1 have been identified as tumor-associated antigens whose formation is initiated by the Lc3-synthase. Until now, high-affinity IgG monoclonal antibodies (mAbs) against 3'-isoLM1 and 3',6'-isoLD1, which are highly expressed in gliomas, have not been developed, although mAbs against lacto-series gangliosides are powerful tools for functional studies. We previously produced the Lc3-synthase gene beta3Gn-T5 knockout mice. In this study, we immunized beta3Gn-T5 knockout mice with 3'-isoLM1/3',6'-isoLD1 and produced the anti-3'-isoLM1/3',6'-isoLD1 mAb GMab-1, of the IgG(3) subclass, which should be useful for functional analysis of lacto-series gangliosides and for antibody-based therapy of gliomas.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Imunoglobulina G
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Biomarcadores Tumorais
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Gangliosídeos
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Glioma
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Anticorpos Monoclonais
Limite:
Animals
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article