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Synthesis of gamma-aminobutyric acid (GABA) by Lactobacillus plantarum DSM19463: functional grape must beverage and dermatological applications.
Di Cagno, Raffaella; Mazzacane, Francesco; Rizzello, Carlo Giuseppe; De Angelis, Maria; Giuliani, Giammaria; Meloni, Marisa; De Servi, Barbara; Gobbetti, Marco.
Afiliação
  • Di Cagno R; Dipartimento di Protezione delle Piante e Microbiologia Applicata, Facoltà di Agraria, Università degli Studi di Bari, Via G. Amendola 165/a, 70126, Bari, Italy.
Appl Microbiol Biotechnol ; 86(2): 731-41, 2010 Mar.
Article em En | MEDLINE | ID: mdl-20013120
Agriculture surplus were used as substrates to synthesize gamma-aminobutyric acid (GABA) by Lactobacillus plantarum DSM19463 for the manufacture of a functional beverage or as a novel application for dermatological purposes. Dilution of the grape must to 1 or 4% (w/v) of total carbohydrates favored higher cell yield and synthesis of GABA with respect to whey milk. Optimal conditions for synthesizing GABA in grape must were: initial pH 6.0, initial cell density of Log 7.0 cfu/mL, and addition of 18.4 mM L-glutamate. L. plantarum DSM19463 synthesized 4.83 mM of GABA during fermentation at 30 degrees C for 72 h. The fermented grape must also contain various levels of niacin, free minerals, and polyphenols, and Log 10.0 cfu/g of viable cells of L. plantarum DSM19463. Freeze dried preparation of grape must was applied to the SkinEthic(R) Reconstructed Human Epidermis or multi-layer human skin model (FT-skin tissue). The effect on transcriptional regulation of human beta-defensin-2 (HBD-2), hyaluronan synthase (HAS1), filaggrin (FGR), and involucrin genes was assayed through RT-PCR. Compared to GABA used as pure chemical compound, the up-regulation HBD-2 was similar while the effect on the expression of HAS1 and FGR genes was higher.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Bebidas / Neurotransmissores / Vitis / Lactobacillus plantarum / Ácido gama-Aminobutírico Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2010 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Bebidas / Neurotransmissores / Vitis / Lactobacillus plantarum / Ácido gama-Aminobutírico Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Ano de publicação: 2010 Tipo de documento: Article