Coordinated actions of actin and BAR proteins upstream of dynamin at endocytic clathrin-coated pits.
Dev Cell
; 17(6): 811-22, 2009 Dec.
Article
em En
| MEDLINE
| ID: mdl-20059951
ABSTRACT
The GTPase dynamin, a key player in endocytic membrane fission, interacts with numerous proteins that regulate actin dynamics and generate/sense membrane curvature. To determine the functional relationship between these proteins and dynamin, we have analyzed endocytic intermediates that accumulate in cells that lack dynamin (derived from dynamin 1 and 2 double conditional knockout mice). In these cells, actin-nucleating proteins, actin, and BAR domain proteins accumulate at the base of arrested endocytic clathrin-coated pits, where they support the growth of dynamic long tubular necks. These results, which we show reflect the sequence of events in wild-type cells, demonstrate a concerted action of these proteins prior to, and independent of, dynamin and emphasize similarities between clathrin-mediated endocytosis in yeast and higher eukaryotes. Our data also demonstrate that the relationship between dynamin and actin is intimately connected to dynamin's endocytic role and that dynamin terminates a powerful actin- and BAR protein-dependent tubulating activity.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Invaginações Revestidas da Membrana Celular
/
Dinamina II
Limite:
Animals
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article