Purifying natively folded proteins from inclusion bodies using sarkosyl, Triton X-100, and CHAPS.
Biotechniques
; 48(1): 61-4, 2010 Jan.
Article
em En
| MEDLINE
| ID: mdl-20078429
ABSTRACT
We describe a rapid, simple, and efficient method for recovering glutathione S-transferase (GST)- and His6-tagged maltose binding protein (MBP) fusion proteins from inclusion bodies. Incubation of inclusion bodies with 10% sarkosyl effectively solubilized >95% of proteins, while high-yield recovery of sarkosyl-solubilized fusion proteins was obtained with a specific ratio of Triton X-100 and CHAPS. We demonstrate for the first time that this combination of three detergents significantly improves binding efficiency of GST and GST fusion proteins to gluthathione (GSH) Sepharose.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes de Fusão
/
Corpos de Inclusão
/
Proteínas Periplásmicas de Ligação
/
Glutationa Transferase
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article