Platform for in situ real-time measurement of protein-induced conformational changes of DNA.
Proc Natl Acad Sci U S A
; 107(4): 1397-401, 2010 Jan 26.
Article
em En
| MEDLINE
| ID: mdl-20080702
ABSTRACT
A platform for in situ and real-time measurement of protein-induced conformational changes in dsDNA is presented. We combine electrical orientation of surface-bound dsDNA probes with an optical technique to measure the kinetics of DNA conformational changes. The sequence-specific Escherichia coli integration host factor is utilized to demonstrate protein-induced bending upon binding of integration host factor to dsDNA probes. The effects of probe surface density on binding/bending kinetics are investigated. The platform can accommodate individual spots of microarrayed dsDNA on individually controlled, lithographically designed electrodes, making it amenable for use as a high throughput assay.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
DNA Bacteriano
/
Sondas de DNA
/
Proteínas de Escherichia coli
/
Escherichia coli
/
Conformação de Ácido Nucleico
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article