Stimulation and inhibition of 5 ALA induced PplX fluorescence in the diagnosis of fibrosarcoma cultivated on the CAM using glucose versus ethanol as modulating agents.

The fluorescence properties of biological tissues have been considered as intrinsic parameters to discriminate diseased from normal conditions. In vivo fluorescence diagnosis of cancer is based on special fluorescent dyes and their properties of tumour selective retention. The experimental in vivo model of the chorioallantoic membrane (CAM) of chicken embryos was used for cultivating a murine tumourous system consisting of the SSK II fibrosarcoma. Proto porphyrine (PplX) synthesis in CAM inoculated tissues as well as in native CAM was induced by 5-ALA. The modulation effects of several biochemicals on the 5-ALA induced PplX production were tested. The fibrosarcoma cells have not revealed autofluorescence with distinctively higher signal intensities than the substrate tissue. Fibrosarcoma cells are clearly distinguished by higher xenofluorescence intensities compared to the CAM tissue in the background. 5-ALA induced xenofluorescence intensity in fibrosarcoma was significantly enhanced by glucose and inhibited by ethanol. It can be concluded that some chemical agents can modulate the intensity of 5-ALA induced xenofluorescence through their modulation the enzymatic cell activity and these can be used for improvement by varying both the diagnostic and the therapeutic effectiveness of the photosensitizers in its application in the photo therapy process.