Visualizing "green oil" in live algal cells.

Cooper, Mark Scott; Hardin, William Robert; Petersen, Timothy Wayne; Cattolico, Rose Ann

Cooper, Mark Scott; Hardin, William Robert; Petersen, Timothy Wayne; Cattolico, Rose Ann.

Afiliação

Cooper MS; Department of Biology, University of Washington, Seattle, WA 98195-1800, USA. mscooper@u.washington.edu

J Biosci Bioeng ; 109(2): 198-201, 2010 Feb.

Article em En | MEDLINE | ID: mdl-20129108

ABSTRACT

ABSTRACT

We report here that BODIPY 505/515, a green lipophilic fluorescent dye, serves as an excellent vital stain for the oil-containing lipid bodies of live algal cells. BODIPY 505/515 vital staining can be used in combination with fluorescent activated cell sorting to detect and isolate algal cells possessing high lipid content.

Assuntos

Compostos de Boro/química; Eucariotos/citologia; Corantes Fluorescentes/química; Eucariotos/química; Citometria de Fluxo; Microscopia Confocal

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Compostos de Boro / Eucariotos / Corantes Fluorescentes Idioma: En Ano de publicação: 2010 Tipo de documento: Article

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