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Impact of varicella-zoster virus on dendritic cell subsets in human skin during natural infection.
Huch, Jennifer H; Cunningham, Anthony L; Arvin, Ann M; Nasr, Najla; Santegoets, Saskia J A M; Slobedman, Eric; Slobedman, Barry; Abendroth, Allison.
Afiliação
  • Huch JH; University of Sydney, Discipline of Infectious Diseases and Immunology, Blackburn Building, Room 601, New South Wales 2006, Australia.
J Virol ; 84(8): 4060-72, 2010 Apr.
Article em En | MEDLINE | ID: mdl-20130046
ABSTRACT
Varicella-zoster virus (VZV) causes varicella and herpes zoster, diseases characterized by distinct cutaneous rashes. Dendritic cells (DC) are essential for inducing antiviral immune responses; however, the contribution of DC subsets to immune control during natural cutaneous VZV infection has not been investigated. Immunostaining showed that compared to normal skin, the proportion of cells expressing DC-SIGN (a dermal DC marker) or DC-LAMP and CD83 (mature DC markers) were not significantly altered in infected skin. In contrast, the frequency of Langerhans cells was significantly decreased in VZV-infected skin, whereas there was an influx of plasmacytoid DC, a potent secretor of type I interferon (IFN). Langerhans cells and plasmacytoid DC in infected skin were closely associated with VZV antigen-positive cells, and some Langerhans cells and plasmacytoid DC were VZV antigen positive. To extend these in vivo observations, both plasmacytoid DC (PDC) isolated from human blood and Langerhans cells derived from MUTZ-3 cells were shown to be permissive to VZV infection. In VZV-infected PDC cultures, significant induction of alpha IFN (IFN-alpha) did not occur, indicating the VZV inhibits the capacity of PDC to induce expression of this host defense cytokine. This study defines changes in the response of DC which occur during cutaneous VZV infection and implicates infection of DC subtypes in VZV pathogenesis.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Pele / Células Dendríticas / Varicela / Herpesvirus Humano 3 / Herpes Zoster Limite: Humans Idioma: En Ano de publicação: 2010 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Pele / Células Dendríticas / Varicela / Herpesvirus Humano 3 / Herpes Zoster Limite: Humans Idioma: En Ano de publicação: 2010 Tipo de documento: Article