Use of the kinase inhibitor analog 1NM-PP1 reveals a role for Toxoplasma gondii CDPK1 in the invasion step.

Sugi, Tatsuki; Kato, Kentaro; Kobayashi, Kyosuke; Watanabe, Shumpei; Kurokawa, Hitomi; Gong, Haiyan; Pandey, Kishor; Takemae, Hitoshi; Akashi, Hiroomi

Sugi, Tatsuki; Kato, Kentaro; Kobayashi, Kyosuke; Watanabe, Shumpei; Kurokawa, Hitomi; Gong, Haiyan; Pandey, Kishor; Takemae, Hitoshi; Akashi, Hiroomi.

Afiliação

Sugi T; Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.

Eukaryot Cell ; 9(4): 667-70, 2010 Apr.

Article em En | MEDLINE | ID: mdl-20173034

ABSTRACT

ABSTRACT

Toxoplasma gondii CDPK1 (TgCDPK1) was found to be the target of the toxoplasmocidal compound 1NM-PP1. When TgCDPK1 was mutated at position 128 from glycine to methionine, resistance was gained. Inhibition of gliding motility without inhibition of micronemal secretion by 1NM-PP1 suggests a function for TgCDPK1 in gliding motility.

Assuntos

Movimento Celular/efeitos dos fármacos; Inibidores de Proteínas Quinases/farmacologia; Proteínas Quinases/metabolismo; Proteínas de Protozoários/metabolismo; Pirazóis/farmacologia; Pirimidinas/farmacologia; Toxoplasma/efeitos dos fármacos; Toxoplasma/fisiologia; Sequência de Aminoácidos; Animais; Feminino; Humanos; Dados de Sequência Molecular; Gravidez; Proteínas Quinases/genética; Proteínas de Protozoários/genética; Alinhamento de Sequência

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas Quinases / Pirazóis / Pirimidinas / Toxoplasma / Proteínas de Protozoários / Movimento Celular / Inibidores de Proteínas Quinases Limite: Animals / Female / Humans / Pregnancy Idioma: En Ano de publicação: 2010 Tipo de documento: Article

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