A combined method for producing homogeneous glycoproteins with eukaryotic N-glycosylation.
Nat Chem Biol
; 6(4): 264-6, 2010 Apr.
Article
em En
| MEDLINE
| ID: mdl-20190762
ABSTRACT
We describe a new method for producing homogeneous eukaryotic N-glycoproteins. The method involves the engineering and functional transfer of the Campylobacter jejuni glycosylation machinery in Escherichia coli to express glycosylated proteins with the key GlcNAc-Asn linkage. The bacterial glycans were then trimmed and remodeled in vitro by enzymatic transglycosylation to fulfill a eukaryotic N-glycosylation. It provides a potentially general platform for producing eukaryotic N-glycoproteins.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Glicosilação
/
Glicoproteínas
/
Engenharia Genética
/
Eucariotos
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article