Crystallization and preliminary crystallographic analysis of mouse peroxiredoxin II with significant pseudosymmetry.
Acta Crystallogr Sect F Struct Biol Cryst Commun
; 66(Pt 3): 357-60, 2010 Mar 01.
Article
em En
| MEDLINE
| ID: mdl-20208180
ABSTRACT
Peroxiredoxin II was cloned from mouse B cells into pCold 1 expression vector and produced as a His-tagged recombinant protein in Escherichia coli. A ring form was isolated by gel filtration. A crystal obtained by the sitting-drop vapour-diffusion method diffracted to 1.77 A resolution at 100 K. The crystal belonged to space group P2(1)2(1)2, with unit-cell parameters a = 117.4, b = 133.9, c = 139.1 A. The asymmetric unit is expected to contain six dimers of peroxiredoxin II, with a corresponding solvent content of 39.3%. Peaks in the native Patterson function together with pseudo-systematic absences suggested that the crystals suffered from severe translational pseudosymmetry.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peroxirredoxinas
Limite:
Animals
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article