Attenuating ischemia-induced H9c2 myoblasts apoptosis by therapeutic hypothermia.
Am J Med Sci
; 339(3): 258-65, 2010 Mar.
Article
em En
| MEDLINE
| ID: mdl-20220335
ABSTRACT
Although inducing mild hypothermia (32 degrees C) in animal models of cardiac arrest with highly attenuated cardiac and neurological injury, the protective effects of hypothermia molecular mechanisms were not fully elucidated, and thus, were examined here on the H9c2 rat ventricular myoblasts that underwent cell loss as well as apoptosis in conditions of simulated ischemia, represented by serum withdrawal plus hypoxia. The H9c2 cells apoptosis was evidenced by flow cytometry-, DNA fragmentation-, and caspase 3 activation-increased apoptotic cells (Annexin-V positive and propidium iodide negative). For the simulated ischemia, both cell loss and apoptosis of these cardiomyoblasts were associated with downregulated small molecular weight proteins (heat shock protein 20, heat shock protein 27, and alphaB-crystallin). Mild hypothermia significantly reduced the ischemia-induced apoptosis, small molecular weight proteins downregulation, and cell viability cut. Conclusively, hypothermia may inhibit simulated ischemia-induced apoptosis in cardiomyocytes by restoring normal small molecular weight proteins expression.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Apoptose
/
Mioblastos
/
Hipotermia Induzida
Limite:
Animals
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article