The extensin signal peptide allows secretion of a heterologous protein from protoplasts.
Gene
; 99(1): 95-100, 1991 Mar 01.
Article
em En
| MEDLINE
| ID: mdl-2022327
ABSTRACT
Extensins are hydroxyproline-rich glycoproteins which are amongst the most abundant proteins present in the cell wall of higher plants. Here, we describe the structural analysis of an extensin-encoding gene from Nicotiana plumbaginifolia. The encoded protein (46 kDa) has a highly repetitive structure and contains 37% proline, 18.1% tyrosine, 13.4% lysine, 8.1% serine and 7.1% histidine. The extensin-encoding sequence contains a typical signal peptide for translocation of the protein to the endoplasmic reticulum. By using chimeric genes consisting of different 5' parts of the extensin-encoding gene and the neomycin phosphotransferase II-encoding gene (nptII) as reporter gene, we show that the N-terminal part of extensin can mediate the secretion of NPTII from electroporated N. tabacum protoplasts.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Plantas
/
Plantas Tóxicas
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Nicotiana
/
Sinais Direcionadores de Proteínas
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Glicoproteínas
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Genes de Plantas
Idioma:
En
Ano de publicação:
1991
Tipo de documento:
Article