[Determination of ethyl glucuronide in blood by GC-MS/MS].

ABSTRACT

OBJECTIVE: To develop a method for determining ethyl glucuronide(EtG) in human blood with gas chromatograph-tandem mass spectrometry (GC-MS/MS). METHODS: Human blood protein was precipitated with acetonitrile. The supernatant was transferred and air flow dried after centrifugated. The residue was derived with N, O-bis (trimethylsilyl)trifluoroacetamide (BSTFA), and analyzed with GC-MS/MS. RESULTS: The detection limit of EtG in blood was 0.05 microg/mL. Calibration curve covered a span from 0.1-10 microg/mL with a good linear relationship (r = 0.999 9). The method showed a excellent performance when was used to authentic blood sample analysis for EtG. CONCLUSION: The method is suitable for blood EtG analysis.