Exercise linked to transient increase in expression and activity of cation channels in newly formed hind-limb collaterals.
Eur J Vasc Endovasc Surg
; 40(1): 81-7, 2010 Jul.
Article
em En
| MEDLINE
| ID: mdl-20304685
ABSTRACT
OBJECTIVE:
This study aimed to compare arteriogenesis after femoral artery occlusion as influenced by exercise or arteriovenous shunt and follow changes in collateral transient receptor potential cation channel, subfamily V, member 4 (Trpv4).DESIGN:
A prospective, controlled study wherein rats were subjected to femoral artery ligation (FAL), or FAL+arteriovenous shunt. Collateral Trpv4 was determined 0.5 and 6h post exercise.METHODS:
Rats were subjected to exercise for 15 min, twice daily. The number and diameter of collaterals were assessed after 7 days. Collateral Trpv4 expression was quantified by reverse transcription-polymerase chain reaction.RESULTS:
Collateral number and diameter per limb were significantly higher in the shunt group (number 16.0+/-2.4 and diameter 216.0+/-34 microm) compared to the ligature (number 9.4+/-2 and diameter 144+/-21 microm) and exercise groups (number 9.9+/-2.5 and diameter 151+/-15 microm). Trpv4 expression in collaterals harvested 0.5h post exercise was not significantly different from expression in shunted rats. It was significantly lower in collaterals harvested 6h post exercise (comparable to that in ligated rats).CONCLUSION:
Collateral formation was greater in the shunt group than in the exercise group. Exercise-induced Trpv4 up-regulation, not significantly different from that achieved with shunt, returned to control values when evaluated 6h post exercise. More frequent exercise to chronically increase fluid shear stress, as with a shunt model, may be required for sufficient arteriogenesis to compensate for peripheral occlusion.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Condicionamento Físico Animal
/
Arteriopatias Oclusivas
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Circulação Colateral
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Músculo Esquelético
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Canais de Cátion TRPV
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article