The random-model method enables ab initio 3D reconstruction of asymmetric particles and determination of particle symmetry.
J Struct Biol
; 171(2): 216-22, 2010 Aug.
Article
em En
| MEDLINE
| ID: mdl-20353825
ABSTRACT
Model-based, 3D reconstruction techniques depend on reliable starting models. We present an extension of the random-model method (RMM) that allows the ab initio generation of suitable starting models directly from un-averaged, experimental images of asymmetric or symmetric particles. Therefore, the asymmetric RMM can also be used to determine point-group symmetry. The procedure is facilitated by the use of (a) variable angular step-sizes during iterative origin and orientation searches, (b) high numbers of particle images, and (c) highly defocused images. The method is inhibited by mixed-handedness orientation assignments and by particles with inconspicuous features. For symmetric particles, symmetric RMMs can overcome these deficiencies.
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1
Base de dados:
MEDLINE
Assunto principal:
Processamento de Imagem Assistida por Computador
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Microscopia Crioeletrônica
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Imageamento Tridimensional
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Modelos Teóricos
Tipo de estudo:
Clinical_trials
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Prognostic_studies
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article