Cryogenic X-ray diffraction microscopy for biological samples.
Phys Rev Lett
; 103(19): 198102, 2009 Nov 06.
Article
em En
| MEDLINE
| ID: mdl-20365956
ABSTRACT
X-ray diffraction microscopy (XDM) is well suited for nondestructive, high-resolution biological imaging, especially for thick samples, with the high penetration power of x rays and without limitations imposed by a lens. We developed nonvacuum, cryogenic (cryo-) XDM with hard x rays at 8 keV and report the first frozen-hydrated imaging by XDM. By preserving samples in amorphous ice, the risk of artifacts associated with dehydration or chemical fixation is avoided, ensuring the imaging condition closest to their natural state. The reconstruction shows internal structures of intact D. radiodurans bacteria in their natural contrast.
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Base de dados:
MEDLINE
Assunto principal:
Difração de Raios X
/
Deinococcus
/
Congelamento
/
Microscopia
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article