Microarray-based detection of CYP1A1, CYP2C9, CYP2C19, CYP2D6, GSTT1, GSTM1, MTHFR, MTRR, NQO1, NAT2, HLA-DQA1, and AB0 allele frequencies in native Russians.
Genet Test Mol Biomarkers
; 14(3): 329-42, 2010 Jun.
Article
em En
| MEDLINE
| ID: mdl-20373852
ABSTRACT
Xenobiotic-metabolizing genes (e.g., Cytochromes P450, GST, NAT2, and NQO1), folate metabolism genes (e.g., MTHFR and MTRR), and major histocompatibility complex genes (e.g., HLA-DQA1) play multiple roles in the organism functioning. In addition, AB0 is the most clinically significant high-polymorphic gene in transfusion and transplantation medicine. Epidemiological data show that allele frequencies of these genes exhibit ethnic and geographic diversity. Besides, little is known about frequency distribution of the major polymorphic variants in native Russians. We developed biological microchips that allow us to analyze a spectrum of allelic variants in 12 different genes CYP1A1, CYP2D6, CYP2C9, CYP2C19, GSTT1, GSTM1, MTHFR, MTRR, NQO1, NAT2, HLA-DQA1, and AB0. Using this composite methodological platform we have studied 352 DNA samples from healthy native Russian volunteers. The allelic frequencies of gene polymorphisms obtained are close to allelic frequencies observed in some European populations, as published earlier. These data were used in comparative studies to determine predisposition to tuberculosis, lymphoma, and leukemia in adults and to childhood acute leukemia. The HLA-DQA1 and AB0 allele frequencies were used to estimate forensic population parameters for these loci.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Oxirredutases
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Sistema ABO de Grupos Sanguíneos
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Antígenos HLA-DQ
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Análise de Sequência com Séries de Oligonucleotídeos
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População Branca
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Frequência do Gene
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Oxigenases de Função Mista
Tipo de estudo:
Diagnostic_studies
Limite:
Adolescent
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Adult
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Female
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Humans
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Male
País como assunto:
Asia
/
Europa
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article