Amplex UltraRed enhances the sensitivity of fluorimetric pyruvate detection.
Anal Biochem
; 403(1-2): 123-5, 2010 Aug.
Article
em En
| MEDLINE
| ID: mdl-20382105
ABSTRACT
Pyruvate, which is produced in the final step of glycolysis, participates in anabolic metabolism, catabolic metabolism, and signal transduction. We have recently reported a new sensitive and selective fluorimetric assay for pyruvate measurement using Amplex Red as a fluorogenic dye. However, the fluorescence of the reaction product, resorufin, is pH dependent, which limits the sensitivity of the assay at pH 6.7. In this Note, we evaluate Invitrogen's new dye, Amplex UltraRed, as a fluorogenic agent for pyruvate determination. Our results show that Amplex UltraRed improves the performance of the assay, providing brighter fluorescence and enhanced sensitivity.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ácido Pirúvico
/
Corantes Fluorescentes
/
Fluorometria
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article