Two-dimensional reference map for the basic proteome of the human dorsolateral prefrontal cortex (dlPFC) of the prefrontal lobe region of the brain.
Proteomics
; 10(13): 2551-5, 2010 Jul.
Article
em En
| MEDLINE
| ID: mdl-20432482
ABSTRACT
We describe a 2-DE proteomic reference map containing 227 basic proteins in the dorsolateral prefrontal cortex region of the human brain. Proteins were separated in the first dimension on pH 6-11 IPG strips using paper-bridge loading and on 12% SDS-PAGE in the second dimension. Proteins were subsequently identified by MS and spectra were analyzed using an in-house proteomics data analysis platform, Proline. The 2-DE reference map is available via the UCD 2-DE Proteome Database (http//proteomics-portal.ucd.ie8082) and can also be accessed via the WORLD-2DPAGE Portal (http//www.expasy.ch/world-2dpage/). The associated protein identification data have been submitted to the PRIDE database (accession numbers 10018-10033). Separation of proteins in the basic region resolves more membrane associated proteins relevant to the synaptic pathology central to many neurological disorders. The 2-DE reference map will aid with further characterisation of neurological disorders such as bipolar and schizophrenia.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Química Encefálica
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Córtex Pré-Frontal
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Proteoma
Limite:
Humans
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article