Direct microfabrication of topographical and chemical cues for the guided growth of neural cell networks on polyamidoamine hydrogels.
Macromol Biosci
; 10(8): 842-52, 2010 Aug 11.
Article
em En
| MEDLINE
| ID: mdl-20437406
ABSTRACT
Cell patterning is an important tool for organizing cells in surfaces and to reproduce in a simple way the tissue hierarchy and complexity of pluri-cellular life. The control of cell growth, proliferation and differentiation on solid surfaces is consequently important for prosthetics, biosensors, cell-based arrays, stem cell therapy and cell-based drug discovery concepts. We present a new electron beam lithography method for the direct and simultaneous fabrication of sub-micron topographical and chemical patterns, on a biocompatible and biodegradable PAA hydrogel. The localized e-beam modification of a hydrogel surface makes the pattern able to adsorb proteins in contrast with the anti-fouling surface. By also exploiting the selective attachment, growth and differentiation of PC12 cells, we fabricated a neural network of single cells connected by neuritis extending along microchannels. E-beam microlithography on PAA hydrogels opens up the opportunity of producing multifunctional microdevices incorporating complex topographies, allowing precise control of the growth and organization of individual cells.
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1
Base de dados:
MEDLINE
Assunto principal:
Hidrogéis
/
Rede Nervosa
/
Nylons
Limite:
Animals
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article