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A novel role for 14-3-3sigma in regulating epithelial cell polarity.
Ling, Chen; Zuo, Dongmei; Xue, Bin; Muthuswamy, Senthil; Muller, William J.
Afiliação
  • Ling C; Department of Biochemistry, McGill University, Montreal, Quebec H3A 1A3, Canada.
Genes Dev ; 24(9): 947-56, 2010 May.
Article em En | MEDLINE | ID: mdl-20439433
The loss of epithelial polarity is thought to play an important role during mammary tumor progression. Using a unique transgenic mouse model of ErbB2-induced mammary tumorigenesis, we demonstrated previously that amplification of ErbB2 is frequently accompanied by loss of the 14-3-3sigma gene. Here, we demonstrate that ectopic expression of 14-3-3sigma results in restoration of epithelial polarity in ErbB2-transformed mammary tumor cells. We further demonstrate that targeted deletion of 14-3-3sigma within primary mammary epithelial cells increases their proliferative capacity and adversely affects their ability to form polarized structures. Finally, we show that 14-3-3sigma can specifically form complexes with Par3, a protein that is essential for the maintenance of a polarized epithelial state. Taken together, these observations suggest that 14-3-3sigma plays a critical role in retaining epithelial polarity.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Polaridade Celular / Proteínas 14-3-3 / Células Epiteliais Limite: Animals Idioma: En Ano de publicação: 2010 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Polaridade Celular / Proteínas 14-3-3 / Células Epiteliais Limite: Animals Idioma: En Ano de publicação: 2010 Tipo de documento: Article