A visual chip-based coimmunoprecipitation technique for analysis of protein-protein interactions.
Anal Biochem
; 404(2): 244-6, 2010 Sep 15.
Article
em En
| MEDLINE
| ID: mdl-20460096
ABSTRACT
Here we report a visual chip-based coimmunoprecipitation (vChip-coIP) platform for analysis of protein-protein interactions (PPIs) by combining advantages of an antibody microarray, traditional coIP, and a silver enhancement detection method. The chip was fabricated by spotting anti-Flag antibody on aldehyde-modified slides, and the resulting platform could assay immunoprecipitate from a small amount of crude cell lysates containing Flag-bait and Myc-prey. The interaction signals are visible using biotinylated anti-Myc antibody and colloidal gold-labeled streptavidin followed by a silver enhancement detection method. It is shown that vChip-coIP is a simple, cost-effective, and highly efficient platform for the comprehensive study of PPIs.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Mapeamento de Interação de Proteínas
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Análise Serial de Proteínas
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Imunoprecipitação
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Anticorpos
Limite:
Humans
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article