Functional rules for lac repressor-operator associations and implications for protein-DNA interactions.
Protein Sci
; 19(6): 1162-72, 2010 Jun.
Article
em En
| MEDLINE
| ID: mdl-20512969
ABSTRACT
The Lac repressor has been used as a tool to understand protein-DNA recognition for many years. Recent experiments have demonstrated the ability of the Lac repressor to control gene expression in various eukaryotic systems, making the quest for an arsenal of protein-DNA binding partners desirable for potential therapeutic applications. Here, we present the results of the most exhaustive screen of Lac repressor-DNA binding partners to date, resulting in the elucidation of functional rules for Lac-DNA binding. Even within the confines of a single protein-DNA scaffold, modes of binding of different protein-DNA partners are sufficiently diverse so as to prevent elucidation of generalized rules for recognition for a single protein, much less an entire protein family.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas Repressoras
/
Proteínas de Escherichia coli
/
Domínios e Motivos de Interação entre Proteínas
/
Repressores Lac
Tipo de estudo:
Prognostic_studies
/
Risk_factors_studies
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article