Effects of Brn-4 on the neuronal differentiation of neural stem cells derived from rat midbrain.
Cell Biol Int
; 34(9): 877-82, 2010 Sep.
Article
em En
| MEDLINE
| ID: mdl-20524937
ABSTRACT
NSCs (neural stem cells) provide a powerful research tool for the design and discovery of new approaches to cell replacement therapy during brain repair. However, the usefulness of this tool has been particularly obstructed by limited neuronal differentiation of NSCs. Brn-4, a member of the POU domain family of transcription factors, has been previously implicated in the development of neurons by expression analysis. Here, we directly investigated the effects of Brn-4 on the neuronal differentiation and development of NSCs derived from the E13 rat midbrain. We found that Brn-4 knockdown in NSCs resulted in a significant decrease of MAP-2-positive neurons with immature morphology. Overexpression of Brn-4 in NSCs markedly increased the production and maturation of newborn neurons. These results suggest that Brn-4 has a critical role in the neuronal differentiation of mesencephalic NSCs and the maturation of newborn neurons. Brn-4 may be utilized to manipulate NSCs for gene and cell therapy of several neurological diseases.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco
/
Mesencéfalo
/
Diferenciação Celular
/
Fatores do Domínio POU
/
Proteínas do Tecido Nervoso
/
Neurônios
Limite:
Animals
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article