Molecular characterization and heterologous expression of quinate dehydrogenase gene from Gluconobacter oxydans IFO3244.
Biochemistry (Mosc)
; 75(4): 452-9, 2010 Apr.
Article
em En
| MEDLINE
| ID: mdl-20618134
ABSTRACT
The quinate dehydrogenase (QDH) from Gluconobacter oxydans IFO3244 exhibits high affinity for quinate, suggesting its application in shikimate production. Nucleotide sequence analysis of the qdh gene revealed a full-length of 2475-bp encoding an 824-amino acid protein. The qdh gene has the unusual TTG translation initiation codon. Conserved regions and a signature sequence for the quinoprotein family were observed. Phylogenetic analysis demonstrated relatedness of QDH from G. oxydans to other quinate/shikimate dehydrogenases with the highest similarity (56%) with that of Acinetobacter calcoaceticus ADP1 and lower similarity (36%) with a membrane-bound glucose dehydrogenase of Escherichia coli. The function of the gene coding for QDH was confirmed by heterologous gene expression in pyrroloquinoline quinone-synthesizing Pseudomonas putida HK5.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Gluconobacter oxydans
/
Oxirredutases do Álcool
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article