Luminol encapsulated liposome as a signal generator for the detection of specific antigen-antibody reactions and nucleotide hybridization.
Anal Sci
; 26(7): 767-72, 2010.
Article
em En
| MEDLINE
| ID: mdl-20631437
ABSTRACT
Liposomes prepared with biotinylated phospholipids and luminol entrapped were shown to be of 187 nm in size, 59% of which were unilamellar and with 43% luminol trapping efficiency. Liposome prepared from biotinylated phospholipids with a longer hydrophilic PEG2000 spacer, but not with the shorter hydrophobic caproyl one, bound efficiently and specifically with immobilized streptavidin in a microplate assay. The interactions of dinitrophenol and tobramycin with their respective antibodies, and the hybridization of 20-mers oligonucleotides were studied using the liposome as a signal generator. These reactions were shown to be specific with limits of detection of 0.58 microM, 0.96 microM and 18 nM, respectively.
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Base de dados:
MEDLINE
Assunto principal:
Oligonucleotídeos
/
Técnicas Biossensoriais
/
Lipossomos
/
Luminol
/
Especificidade de Anticorpos
/
Complexo Antígeno-Anticorpo
/
Reações Antígeno-Anticorpo
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article