Cellular source and molecular form of TNF specify its distinct functions in organization of secondary lymphoid organs.
Blood
; 116(18): 3456-64, 2010 Nov 04.
Article
em En
| MEDLINE
| ID: mdl-20634375
ABSTRACT
Secondary lymphoid organs provide a unique microenvironment for generation of immune responses. Using a cell type-specific conditional knockout approach, we have dissected contributions of tumor necrosis factor (TNF) produced by B cells (B-TNF) or T cells (T-TNF) to the genesis and homeostatic organization of secondary lymphoid organs. In spleen, lymph nodes and Peyer patches, the cellular source of TNF, and its molecular form (soluble versus membrane-bound) appeared distinct. In spleen, in addition to major B-TNF signal, a complementary T-TNF signal contributed to the microstructure. In contrast, B-TNF predominantly controlled the development of follicular dendritic cells and B-cell follicles in Peyer patches. In lymph nodes, cooperation between TNF expressed by B and T cells was necessary for the maintenance of microarchitecture and for generation of an efficient humoral immune response. Unexpectedly, soluble but not membrane TNF expressed by B cells was essential for the organization of the secondary lymphoid organs. Thus, the maintenance of each type of secondary lymphoid organ is orchestrated by distinct contributions of membrane-bound and soluble TNF produced by B and T lymphocytes.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Nódulos Linfáticos Agregados
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Baço
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Linfócitos B
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Linfócitos T
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Fator de Necrose Tumoral alfa
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Linfonodos
Limite:
Animals
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article