H-NS silences gfp, the green fluorescent protein gene: gfpTCD is a genetically Remastered gfp gene with reduced susceptibility to H-NS-mediated transcription silencing and with enhanced translation.
J Bacteriol
; 192(18): 4790-3, 2010 Sep.
Article
em En
| MEDLINE
| ID: mdl-20639321
ABSTRACT
The bacterial nucleoid-associated protein H-NS, which preferentially targets and silences A+T-rich genes, binds the ubiquitous reporter gene gfp and dramatically reduces local transcription. We have redesigned gfp to reduce H-NS-mediated transcription silencing and simultaneously improve translation in vivo without altering the amino acid sequence of the GFP protein.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Transcrição Gênica
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Proteínas de Fluorescência Verde
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Proteínas de Ligação a DNA
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article