Selective diffusion barriers separate membrane compartments.
Biophys J
; 99(1): L1-3, 2010 Jul 07.
Article
em En
| MEDLINE
| ID: mdl-20655822
ABSTRACT
We have investigated exchange of molecules between different membrane domains on a highly compartmentalized cell, the spermatozoon. Using Alexa Fluor 555-cholera toxin B-subunit we have observed clustering of preexisting GM1 gangliosides which diffused across the anterior acrosome-equatorial segment interface but did not access the postacrosome. By contrast, single lipid and protein molecules readily exchanged between all three domains, although they diffused more slowly on nearing and crossing to the postacrosome. Thus, two types of diffusion interfaces are present on sperm heads, an "open" interface and a "mass filter" interface. The latter seems to be due to a protein-cytoskeleton network.
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Base de dados:
MEDLINE
Assunto principal:
Espermatozoides
/
Microdomínios da Membrana
Limite:
Animals
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article