Gene targeting by homologous recombination in mouse zygotes mediated by zinc-finger nucleases.
Proc Natl Acad Sci U S A
; 107(34): 15022-6, 2010 Aug 24.
Article
em En
| MEDLINE
| ID: mdl-20686113
Gene targeting by homologous recombination in embryonic stem cells is extensively used to generate specific mouse mutants. However, most mammalian species lack tools for targeted gene manipulation. Since double-strand breaks strongly increase the rate of homologous recombination at genomic loci, we explored whether gene targeting can be directly performed in zygotes by the use of zinc-finger nucleases. Here we report that gene targeting is achieved in 1.7-4.5% of murine one-cell embryos upon the coinjection of targeting vectors with zinc-finger nucleases, without preselection. These findings enable the manipulation of the mammalian germ line in a single step in zygotes, independent of ES cells.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Zigoto
/
Marcação de Genes
/
Endonucleases
Limite:
Animals
/
Pregnancy
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article