Human spermidine synthase gene: structure and chromosomal localization.
DNA Cell Biol
; 10(6): 467-74, 1991.
Article
em En
| MEDLINE
| ID: mdl-2069720
ABSTRACT
The human spermidine synthase (EC 2.5.1.16) gene was isolated from a genomic library constructed with DNA obtained from a human immunoglobulin G (IgG) myeloma cell line. Subsequent sequence analyses revealed that the gene comprised of 5,818 nucleotides from the cap site to the last A of the putative polyadenylation signal with 8 exons and 7 intervening sequences. The 5'-flanking region of the gene was extremely GC rich, lacking any TATA box but containing CCAAT consensus sequences. No perfect consensus sequence for the cAMP-responsive element for the AP-1 binding site was found, yet the gene contained seven AP-2 binding site consensus sequences. The putative polyadenylation signal was an unusual AATACA instead of AATAAA. Polymerase chain reaction analysis with DNA obtained from human x hamster somatic cell hybrids indicated that human spermidine synthase genomic sequences segregate with human chromosome 1. Transfection of the genomic clone into Chinese hamster ovary cells displaying a low endogenous spermidine synthase activity revealed that the gene was transiently expressed and hence in all likelihood represents a functional gene.
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Base de dados:
MEDLINE
Assunto principal:
Espermidina Sintase
/
Cromossomos Humanos Par 1
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
1991
Tipo de documento:
Article