Current inhibition of human EAG1 potassium channels by the Ca2+ binding protein S100B.
FEBS Lett
; 584(18): 3896-900, 2010 Sep 24.
Article
em En
| MEDLINE
| ID: mdl-20708613
ABSTRACT
Voltage-dependent human ether à go-go (hEAG1) potassium channels are implicated in neuronal signaling as well as in cancer cell proliferation. Unique sensitivity of the channel to intracellular Ca(2+) is mediated by calmodulin (CaM) binding to the intracellular N- and C-termini of the channel. Here we show that application of the acidic calcium-binding protein S100B to inside-out patches of Xenopus oocytes causes Ca(2+)-dependent inhibition of expressed hEAG1 channels. Protein pull-down assays and fluorescence correlation spectroscopy (FCS) revealed that S100B binds to hEAG1 and shares the same binding sites with CaM. Thus, S100B is a potential alternative calcium sensor for hEAG1 potassium channels.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas S100
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Cálcio
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Canais de Potássio Éter-A-Go-Go
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Fatores de Crescimento Neural
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article