Gene expression enabling synthetic diversification of natural products: chemogenetic generation of pacidamycin analogs.
J Am Chem Soc
; 132(35): 12243-5, 2010 Sep 08.
Article
em En
| MEDLINE
| ID: mdl-20712319
ABSTRACT
Introduction of prnA, the halogenase gene from pyrrolnitrin biosynthesis, into Streptomyces coeruleorubidus resulted in efficient in situ chlorination of the uridyl peptide antibotic pacidamycin. The installed chlorine provided a selectably functionalizable handle enabling synthetic modification of the natural product using mild cross-coupling conditions in crude aqueous extracts of the culture broth.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Oxirredutases
/
Nucleosídeos de Pirimidina
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Fatores Biológicos
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Regulação Enzimológica da Expressão Gênica
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Antibacterianos
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article