[Evaluation of the accuracy of domestic commercial HBV DNA real-time polymerase chain reaction kits using COBAS TaqMan HBV Test as reference].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
; 23(6): 479-81, 2009 Dec.
Article
em Zh
| MEDLINE
| ID: mdl-20718363
ABSTRACT
OBJECTIVE:
To evaluate of the accuracy of domestic commercial HBV DNA real-time polymerase chain reaction kits.METHODS:
Using COBAS TaqMan HBV Test as reference, we evaluate the accuracy of a domestic commercial HBV DNA real-time polymerase chain reaction kit (PG).RESULTS:
Among the samples with viral load at the range of 10(1), 10(2), 10(3), 10(4), 10(5), 10(6), 10(7) (IU/mL), the Coefficient of Correlation(r) between the result determined by domestic kit (PG) and those of Roche COBAS TaqMan HBV Test were -0.08011, -0.05056, 0.105642, 0.312181, 0.908046, 0.866175, -0.23295, respectively; the percentage of false negative results were 60%, 30%, 33.3%, 8.3%, 0, 0, 0, respectively. Among the samples with viral load over than 10(7) (IU/ml), the result determined by PG is significantly lower.CONCLUSION:
The accuracy of PG is not satisfied, especially in those samples with viral load less than 10(4) (IU/ml). A implication from these observation is that samples from patients received antiviral treatment should be tested by Roche COBAS TaqMan HBV Test.
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Eixos temáticos:
Pesquisa_clinica
Base de dados:
MEDLINE
Assunto principal:
Kit de Reagentes para Diagnóstico
/
Vírus da Hepatite B
/
Reação em Cadeia da Polimerase
/
Hepatite B
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Limite:
Humans
País como assunto:
Asia
Idioma:
Zh
Ano de publicação:
2009
Tipo de documento:
Article