An antibiotic selection marker for nematode transgenesis.
Nat Methods
; 7(9): 721-3, 2010 Sep.
Article
em En
| MEDLINE
| ID: mdl-20729841
ABSTRACT
We have developed a nematode transformation vector carrying the bacterial neomycin resistance gene (NeoR) and shown that it could confer resistance to G-418 on both wild-type Caenorhabditis elegans and C. briggsae. This selection system allows hands-off maintenance and enrichment of transgenic worms carrying non-integrated transgenes on selective plates. We also show that this marker can be used for Mos1-mediated single-copy insertion in wild-type genetic backgrounds (MosSCI-biotic).
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Seleção Genética
/
Caenorhabditis
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Resistência a Medicamentos
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Gentamicinas
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Transgenes
Limite:
Animals
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article