Preparative separation of pyrogens from proteins by isoelectric focusing using a multicompartment electrolyser with Immobiline membranes.
Electrophoresis
; 11(11): 981-2, 1990 Nov.
Article
em En
| MEDLINE
| ID: mdl-2079048
ABSTRACT
Due to the nature of lipopolysaccharide endotoxin structures of bacterial pyrogens, their removal from solutions containing therapeutic proteins is often a problem in the pharmaceutical industry. In this report we describe the application of electromotive force to dislodge lipopolysaccharide endotoxins from proteins. This was performed by employing a multicompartment electrolyzer fitted with Immobiline membranes of specified pIs. A thousand-fold reduction of endotoxin could be achieved in the model test system described. This contribution describes the use of a new recycling isoelectric focusing approach without the use of carrier ampholytes.
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Base de dados:
MEDLINE
Assunto principal:
Pirogênios
/
Focalização Isoelétrica
Idioma:
En
Ano de publicação:
1990
Tipo de documento:
Article