[Construction and identification of a vector inserted with complete genome of poliovirus strain Sabin I].

ABSTRACT

OBJECTIVE: To develop a vector inserted with complete genome of poliovirus strain Sabin I. METHODS: The 3 fragments of the complete genome of Sabin I was amplified and cloned to pEASY-T3 by molecular biological technology. These cloned pEASY-T3 were then digested by Restriction enzymes and ligated to pWSK29 step by step and identified. RESULTS: The complete genome of poliovirus strain Sabin I was successfully cloned into vector pWSK29 with 9 nucleotide mutations. CONCLUSION: The complete genome plasmid was constructed and it provided a basis for further research of the function of Sabin I.