Induced biosynthesis of cryptic polyketide metabolites in a Burkholderia thailandensis quorum sensing mutant.
J Am Chem Soc
; 132(40): 13966-8, 2010 Oct 13.
Article
em En
| MEDLINE
| ID: mdl-20853892
ABSTRACT
Genetic manipulation of the LuxR-type quorum sensing regulator system in Burkholderia thailandensis caused a significant change in the metabolic profile it led to activation of the thailandamide biosynthesis gene cluster, dramatically increased thailandamide production, and induced strong pigmentation. A novel polyketide metabolite, thailandamide lactone (2), which cannot be detected in the wild type, was isolated from the mutant broth, and its structure was elucidated by high-resolution mass spectrometry and IR and NMR spectroscopy. In a biological assay using tumor cell lines, 2 showed moderate antiproliferative activities. This finding not only points to complex regulation but also serves as a proof of concept that engineering quorum sensing mutants may enable the discovery of novel bioactive natural products encoded by silent or only weakly expressed biosynthetic pathway genes.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Burkholderia
/
Percepção de Quorum
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article