Characterising B cell numbers and memory B cells in HIV infected and uninfected Malawian adults.
BMC Infect Dis
; 10: 280, 2010 Sep 22.
Article
em En
| MEDLINE
| ID: mdl-20860822
ABSTRACT
BACKGROUND:
Untreated human immunodeficiency virus (HIV) disease disrupts B cell populations causing reduced memory and reduced naïve resting B cells leading to increases in specific co-infections and impaired responses to vaccines. To what extent antiretroviral treatment reverses these changes in an African population is uncertain.METHODS:
A cross-sectional study was performed. We recruited HIV-uninfected and HIV-infected Malawian adults both on and off antiretroviral therapy attending the Queen Elizabeth Central hospital in Malawi. Using flow cytometry, we enumerated B cells and characterized memory B cells and compared these measurements by the different recruitment groups.RESULTS:
Overall 64 participants were recruited - 20 HIV uninfected (HIV-), 30 HIV infected ART naïve (HIV+N) and 14 HIV-infected ART treated (HIV+T). ART treatment had been taken for a median of 33 months (Range 12-60 months). Compared to HIV- the HIV+N adults had low absolute number of naïve resting B cells (111 vs. 180 cells/µl p = 0.008); reduced memory B cells (27 vs. 51 cells/µl p = 0.0008). The HIV+T adults had B-cell numbers similar to HIV- except for memory B cells that remained significantly lower (30 vs. 51 cells/µl p = 0.02). In the HIV+N group we did not find an association between CD4 count and B cell numbers.CONCLUSIONS:
HIV infected Malawian adults have abnormal B-cell numbers. Individuals treated with ART show a return to normal in B-cell numbers but a persistent deficit in the memory subset is noted. This has important implications for long term susceptibility to co-infections and should be evaluated further in a larger cohort study.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Linfócitos B
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Infecções por HIV
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Fármacos Anti-HIV
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Memória Imunológica
Tipo de estudo:
Observational_studies
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Prevalence_studies
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Risk_factors_studies
Limite:
Adult
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Female
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Humans
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Male
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Middle aged
País como assunto:
Africa
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article