[Assessment of the interest of using confirmatory test in ELISA with use of high concentration of heparin-induced thrombocytopenia]. / Évaluation de l'intérêt du test de confirmation avec une forte concentration d'héparine en Elisa dans la thrombopénie induite par l'héparine.
Ann Biol Clin (Paris)
; 68(5): 555-60, 2010.
Article
em Fr
| MEDLINE
| ID: mdl-20870577
ABSTRACT
Heparin-induced thrombocytopenia (HIT) is a severe complication of heparin therapy caused by an antibody against heparin/Platelet Factor 4 (PF4) complex. It complicates the treatment with unfractionated heparin (UFH). The aim of the study was to assess the interest of using confirmatory test in ELISA with use of high concentration of heparin (100 UI/mL) in patients (n=15) with discordance between platelet activation test and ELISA test. We obtained a rate inhibition higher than 50% in 86% of patients was observed. We conclude that inclusion of the high heparin confirmatory procedure in ELISA demonstrate the dependent heparin character of the antibodies but does not improve diagnostic specificity. In addiction a 2-fold increase in final test cost is observed.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Trombocitopenia
/
Ensaio de Imunoadsorção Enzimática
/
Heparina
Tipo de estudo:
Diagnostic_studies
/
Health_economic_evaluation
Limite:
Humans
Idioma:
Fr
Ano de publicação:
2010
Tipo de documento:
Article