Cooperativity between trans and cis interactions in cadherin-mediated junction formation.
Proc Natl Acad Sci U S A
; 107(41): 17592-7, 2010 Oct 12.
Article
em En
| MEDLINE
| ID: mdl-20876147
ABSTRACT
Intercellullar junctions formed by cadherins, including desmosomes and adherens junctions, comprise two dimensional arrays of "trans" dimers formed between monomers emanating from opposing cell surfaces. Lateral "cis" interfaces between cadherins from the same cell surface have been proposed to play a role in cadherin clustering. Although the molecular details of cis interactions remain uncertain, they must define an anisotropic arrangement where binding is favorable only in certain orientations. Here we report Monte Carlo simulations performed on a 2D lattice constructed to account for the anisotropy in cadherin cis interactions. A crucial finding is that the "phase transition" between freely diffusing cadherin monomers and dimers and a condensed ordered 2D junction formed by dimers alone is a cooperative process involving both trans and cis interactions. Moreover, cis interactions, despite being too weak to be measured in solution, are critical to the formation of an ordered junction structure. We discuss these results in light of available experimental information on cadherin binding free energies that are transformed from their bulk solution values to interaction energies on a 2D lattice.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ligação Proteica
/
Modelos Moleculares
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Caderinas
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Junções Intercelulares
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Modelos Químicos
Tipo de estudo:
Health_economic_evaluation
/
Prognostic_studies
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article