The electrochemiluminescence of ruthenium complex/tripropylamine systems at DNA-modified gold electrodes.
Biosens Bioelectron
; 26(5): 2703-6, 2011 Jan 15.
Article
em En
| MEDLINE
| ID: mdl-20880692
ABSTRACT
The electrochemiluminescence (ECL) behavior of ruthenium complex/tripropylamine (TPA) systems at DNA-modified gold electrode was studied to understand the possible mechanism and to develop new detection platforms. DNA strand, especially double-stranded DNA (ds-DNA), can preconcentrate TPA and acts as the acceptor of the protons released from TPAH(+), therefore the improved ECL emission and the low potential ECL were observed. The intercalation of Ru(phen)(3)(2+) into ds-DNA was confirmed to be a sensitive and label-free DNA-related detection platform. The above results were validated by the analysis of lysozyme using anti-lysozyme aptamer-modified electrode. This work opens a new field by the use of DNA-modified electrode to develop novel sensing platforms, such as low potential ECL biosensors and Ru(phen)(3)(2+) intercalation-based ECL biosensors.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Propilaminas
/
Rutênio
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DNA
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Eletrodos
/
Ouro
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Medições Luminescentes
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article