Comparative epigenomic analysis of murine and human adipogenesis.
Cell
; 143(1): 156-69, 2010 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-20887899
ABSTRACT
We report the generation and comparative analysis of genome-wide chromatin state maps, PPARγ and CTCF localization maps, and gene expression profiles from murine and human models of adipogenesis. The data provide high-resolution views of chromatin remodeling during cellular differentiation and allow identification of thousands of putative preadipocyte- and adipocyte-specific cis-regulatory elements based on dynamic chromatin signatures. We find that the specific locations of most such elements differ between the two models, including at orthologous loci with similar expression patterns. Based on sequence analysis and reporter assays, we show that these differences are determined, in part, by evolutionary turnover of transcription factor motifs in the genome sequences and that this turnover may be facilitated by the presence of multiple distal regulatory elements at adipogenesis-dependent loci. We also utilize the close relationship between open chromatin marks and transcription factor motifs to identify and validate PLZF and SRF as regulators of adipogenesis.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Adipogenia
/
Estudo de Associação Genômica Ampla
Tipo de estudo:
Prognostic_studies
Limite:
Adult
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Animals
/
Female
/
Humans
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article