Enzymes that hydrolyze adenine nucleotides in patients with ischemic heart disease.
Clin Chim Acta
; 412(1-2): 159-64, 2011 Jan 14.
Article
em En
| MEDLINE
| ID: mdl-20920495
ABSTRACT
BACKGROUND:
The extracellular nucleotides, ATP and ADP, as well as the nucleoside adenosine have been implicated in a great number of pathologic and physiological functions. However, extracellular adenine nucleotide levels are controlled by a complex cell surface-located group of enzymes called ectonucleotidases. We evaluated activities of enzymes that hydrolyze adenine nucleotides and nucleosides in platelets from patients with ischemic heart disease (IHD).METHODS:
Sixty IHD patients were selected for the study. The activities of ectonucleoside triphosphate diphosphohydrolase (NTPDase, CD39), ectonucleotide pyrophosphatase/phosphodiesterase (E-NPP), ecto-5'-nucleotidase and adenosine deaminase (ADA) were studied in isolated platelets of these patients, as well as the platelet aggregation and NTPDase expression.RESULTS:
The results show that NTPDase, ecto-5'-nucleotidase, E-NPP activities and NTPDase expression were increased in platelets of IHD patients when compared with the control group (p < 0.05). On the other hand, ADA activity and platelet aggregation were decreased in IHD patients, when compared with the control group (p < 0.05).CONCLUSIONS:
The pathological condition in IHD generates alterations in ectonucleotidase activities as a compensatory organic response to thrombotic events that occur in IHD.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Nucleotídeos de Adenina
/
Isquemia Miocárdica
Limite:
Female
/
Humans
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Male
/
Middle aged
Idioma:
En
Ano de publicação:
2011
Tipo de documento:
Article