Neutrophil elastase mediates pathogenic effects of anthrax lethal toxin in the murine intestinal tract.
J Immunol
; 185(9): 5463-7, 2010 Nov 01.
Article
em En
| MEDLINE
| ID: mdl-20921524
ABSTRACT
Neutrophils isolated from BALB/c or C57BL/6 mice and treated in vitro with anthrax lethal toxin release bioactive neutrophil elastase, a proinflammatory mediator of tissue destruction. Similarly, neutrophils isolated from mice treated with anthrax lethal toxin in vivo and cultured ex vivo release greater amounts of elastase than neutrophils from vehicle-treated controls. Direct measurements from murine intestinal tissue samples demonstrate an anthrax lethal toxin-dependent increase in neutrophil elastase activity in vivo as well. These findings correlate with marked lethal toxin-induced intestinal ulceration and bleeding in neutrophil elastase(+/+) animals, but not in neutrophil elastase(-/-) animals. Moreover, neutrophil elastase(-/-) mice have a significant survival advantage over neutrophil elastase(+/+) animals following exposure to anthrax lethal toxin, thereby establishing a key role for neutrophil elastase in mediating the deleterious effects of anthrax lethal toxin.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Toxinas Bacterianas
/
Elastase Pancreática
/
Intestinos
/
Antígenos de Bactérias
/
Neutrófilos
Limite:
Animals
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article